Journal: Journal of Extracellular Biology
Article Title: Dynamics of microRNA secreted via extracellular vesicles during the maturation of embryonic stem cell‐derived retinal pigment epithelium
doi: 10.1002/jex2.70001
Figure Lengend Snippet: Biophysical characterization of sEV secreted from human embryonic stem cell‐derived retinal pigment epithelial cells during maturation. (a) Representative size versus concentration distribution graphs of sEV derived from the three groups of hESC‐RPE cells ( N = 3) as analyzed by NanoSight and representative transmission electron microscopy (TEM) images of sEV providing a visual comparison (scale bar, 20 nm). (b) Distribution and colocalization analysis of tetraspanins (CD81, CD9, and CD63) within sEV derived from the three age groups of hESC‐RPE cells. (c) Results from the MACSPlex assay conducted on early‐, mid‐ , and late‐stage hESC‐RPE‐sEV. Comparisons of: (d) sEV concentration, (e) protein concentration, (f) purity index (particle‐to‐protein ratio), and (g) total RNA and (h) RNA‐to‐sEV particle ratio among the three groups of hESC‐RPE‐sEV. Data are presented as mean ± standard deviation (SD). * p < 0.05, ** p < 0.01.
Article Snippet: sEV were visualized by negative‐stained transmission electron microscopy (TEM) using a JEOL JEM‐2100 microscope mounted on a Gatan OneView IS camera.
Techniques: Derivative Assay, Concentration Assay, Transmission Assay, Electron Microscopy, Comparison, Protein Concentration, Standard Deviation